Composite

Part:BBa_K777112:Design

Designed by: Team Goettingen   Group: iGEM12_Goettingen   (2012-09-22)

fliC under the control of constitutive promoter J23114


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1265
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 342
    Illegal AgeI site found at 750
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

  • The fliC gene contained three PstI sites and one SpeI site that had to be removed. These mutations were induced with different primers via overlap-PCR.


Source

  • The fliC gene was amplified from genomic DNA of E. coli str. K-12 substr. DH10B, complete genome (CP000948.1).
  • J23114 information was taken from the parts.igem and physical DNA from the 2012 distribution kit.

References