Part:BBa_K777109:Design

fliC

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 1224
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 301
  Illegal AgeI site found at 709
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

• The fliC gene contained three PstI sites and one SpeI site that had to be removed. These mutations were induced with different primers via overlap-PCR.

Source

• The part was amplified from genomic DNA of E. coli str. K-12 substr. DH10B, complete genome (CP000948.1).