Measurement

Part:BBa_K772009:Design

Designed by: Mustafa Elitok   Group: iGEM12_Fatih-Medical   (2012-09-26)

GFP - LasR protein complex with flag tags


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1180
    Illegal AgeI site found at 1377
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 693


Design Notes

Note that there is an RBS sequence just before the protein sequence in order to eliminate the need of addition of it. More on that, before the GFP section, there is a flag tag in order to isolate this protein component and use it in the Co-IP assay.

Source

Cleavage site, LasR and GFP sequences are gathered from Registry.

References