Coding

Part:BBa_K769201:Experience

Designed by: Sumiya KAWAI   Group: iGEM12_Tokyo-NoKoGen   (2012-09-19)

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2015 LZU-China

In iGEM 2015, LZU-China used the part as a new reporting gene to monitor the content of pollution. We submitted a twins part BBa_K1755001.

It can increase the electric output by synthesis of riboflavin.

Besides, riboflavin itself is yellow.

After the addition of ribB, the bacteria will be yellow.

We believe that we have construct a new way of measurement by MFC and the part is the crucial device for the measurement.

Usage in MFC

IGEM 2015 MFC.JPG

Microbial Fuel Cells (MFCs) which can convert contaminants in wastewaters into energy is an ideal approach to solve both pollution problem and energy crisis. However, MFCs is hard to determine the contaminants concentrations which is a major drawback for MFCs applications, because of the electric output is too low.

Riboflavin is an excellent electron transport complex. However, the whole sequence of riboflavin synthase is too huge(more than 3000bp) to link to the plasmid pSB1C3. Evidence shows that, part of the riboflavin synthase DNA sequence (ribB) can also improve the secretion of riboflavin.

LZU iGEM 2015 ribB picture.JPG

Figure-1:Normal E.coli(left one)and E.coli with ribB(right one)

K1755006 new.png

Figure-2:The production of riboflavin by overexpression of ribB. This figure shows the data from K1755006(medium promoter +medium RBS + ribB(K1755001 or K769201) + terminators).

We constructed BBa_K1755301 (marO + RBS+ ribB + terminators) to measure the Cu2+ ions in water. We can see the relationship between riboflavin production and Cu2+ concentration.

K1755301ceshi.png

Figure-3 The concentration of riboflavin vs. Cu2+ ions. After incubation of 37h in 37C.

K1755301time.png

Figure-4

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 387
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 277
    Illegal AgeI site found at 319
  • 1000
    COMPATIBLE WITH RFC[1000]


User Reviews

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