Composite

Part:BBa_K769022:Design

Designed by: Tomomi YOKOYAMA   Group: iGEM12_Tokyo-NoKoGen   (2012-09-19)

lux operon(K769011) + GFP(I13522)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
    Illegal NheI site found at 1680
    Illegal NheI site found at 5955
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 6417
    Illegal BamHI site found at 1144
    Illegal XhoI site found at 3767
    Illegal XhoI site found at 4275
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
    Illegal AgeI site found at 1965
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1859
    Illegal BsaI.rc site found at 2541
    Illegal BsaI.rc site found at 9263
    Illegal SapI site found at 961


Design Notes

The construct of Lux operon with GFP is produced by joining the Lux operon and GFP biobrick part.

Bret4.png

Source

Lux operon from Photobacterium phosphoreum

References