Composite
Part:BBa_K769017
Designed by: Mayumi Nakamura Group: iGEM12_Tokyo-NoKoGen (2012-09-19)
Promoter(Pbad)-RBS-luxA-DT-Promoter(high)-RBS-lux CDBFEG-DT
Promoter(Pbad)-RBS-luxA-DT-Promoter(high)-RBS-lux CDBFEG-DT (lux operon from Photobacterium phosphoreum NCMB844)
This part is constructed from our cloned lux operon, BBa_K769010.
It consists of an inducible luxA gene and constitutively epxressed luxCDBEFG gene. We wanted to make the bioluminescent reaction time faster than BBa_K769011. By constitutively expresseing all lux gene except the luxA gene, we thought that the induction of just one gene would shorten the reaction time to observe bioluminescent.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1205
Illegal NheI site found at 2462
Illegal NheI site found at 2485
Illegal NheI site found at 2960
Illegal NheI site found at 6124 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 6586
Illegal BamHI site found at 1144
Illegal XhoI site found at 1360
Illegal XhoI site found at 1868 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 979
Illegal AgeI site found at 3245 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 3139
Illegal BsaI.rc site found at 3821
Illegal SapI site found at 961
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Categories
Parameters
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