Device

Part:BBa_K769000:Design

Designed by: Genki Sakai   Group: iGEM12_Tokyo-NoKoGen   (2012-09-19)

Pconst.(High)-RBS-NpSRII-9a.a.linker_NpHtrII-EnvZ-Double terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 134
    Illegal NgoMIV site found at 392
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1088
    Illegal SapI site found at 862


Design Notes

800px-Rhodopsin2.jpg

Source

SRII and HtrII : Natronomonas pharaonis
EnvZ : E coli

References