Project

Part:BBa_K766004

Designed by: Yu Zhao   Group: iGEM12_Tsinghua   (2012-09-23)

AndNot gate based on AHL

We design this A and not B gate. This part is based on the two kinds of AHL：C6HSL(signal A) and C12HSL(signal B). C6HSL can activate the promoter Plux and C12HSL can activate the promoter Plas. In this part, we add cI operator downstream of Plas. As a result, the promoter Plas can be activated by signal B but repressed by cI protein. The expression of cI protein is under the control of Plux, which can be activated by signal A. RFP is controlled by Hybrid promoter(Plas and cI operator), which will work as a reporter.

In a word, the expression of RFP is only on the condition that A is absent and B is present.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 943
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 385
    Illegal AgeI site found at 582
    Illegal AgeI site found at 1630
    Illegal AgeI site found at 1742
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2859


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