Part:BBa_K763004:Design

pLexA + Gene encoding ZsGreen1

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

No design considerations needed.

Source

The sequence of the promoter comes from E. coli genome, whereas the sequence encoding the AmCyan fluorescent protein is not natural, since it was optimized for codon usage and maximum fluorescence production.

References

1. Radman, B. (1975) "Phenomenology of an inducible mutagenic DNA repair pathway in Escherichia coli: SOS repair hypothesis". Basic Life Sciences 5A: 355–367.
   
2. Michael, B. (2005). "After 30 Years of Study, the Bacterial SOS Response Still Surprises Us". PLoSBiology 3 (7): e255.
   
3. Friedman, N., Vardi, S., Ronen, M., Alon, U. and Stavans J. (2005) Precise Temporal Modulation in the Response of the SOS DNA Repair Network in Individual Bacteria. PLoSBiology 3 (7): e238.
   
4. Cutter, K.L., Alloush, H.M. and Salisbury, V.C. (2007) Stimulation of DNA repair and increased light output in response to UV irradiation in Escherichia coli expressing lux genes. Luminescence 22: 177–181.
   
5. Janion, C. (2008) Inducible SOS Response System of DNA Repair and Mutagenesis in Escherichia coli. Int. J. Biol. Sci. 4.
   
6. Krishna, S., Maslov, S. and Sneppen, K. (2007) UV-Induced Mutagenesis in Escherichia coli SOS Response: A Quantitative Model. PLoSBiology 3 (3): e41.