Reporter
Part:BBa_K763003:Design
Designed by: Pedro Luis Dorado Morales Group: iGEM12_Valencia_Biocampus (2012-09-07)
pGlnA + Gene encoding ZsYellow1
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 689
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
No design considerations needed.
Source
The sequence of the promoter comes from E. coli genome, whereas the sequence encoding the AmCyan fluorescent protein is not natural, since it was optimized for codon usage and maximum fluorescence production.
References
- Reitzer, L.J., Movsas, B. andMagasanik, B. (1989)Activation of glnA transcription by Nitrogen Regulator I (NRI)- phosphate in Escherichia coli: evidence for a long-range physical interaction between NRI-phosphate and RNA polymerase.Journal of bacteriology, 171 (10):5512-5522
- Wang, L., Guo, Y. and Gralla, J.D. (1999) Regulation of sigma 54-dependent transcription by core promoter sequences: role of - 12 region nucleotides. Journal of bacteriology, 181 (24):7558–7565
- Barrios, H., Valderrama, B. y Morett, E. (1999) Compilation and analysis of sigma54-dependent promoter sequences. Nucleics Acids Research, 27 (22):4305-4313.