Part:BBa_K763001:Design
pGroE + Gene encoding AsRed2
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 507
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
No design considerations needed.
Source
The sequence of the promoter comes from E. coli genome, whereas the sequence encoding the AsRed2 fluorescent protein is not natural, since it was optimized for codon usage and maximum fluorescence production.
References
1. De Maio A (1999) Heat shock proteins: facts, thoughts, and dreams. Shock, 11 (1): 1–12. Kusukawa, N., Yura, T., Ueguchi, C., Akiyama, Y. And Ito, K. (1989) Effects of mutations in heat shock genes groES and groEL on protein export in Escherichia coli. EMBO J 8 (11); 3517-21.
2. Nonaka, G., Blankschien, M., Herman, C., Gross, C.A. and Rhodius, V.A. (2006) Regulon and promoter analysis of the E. coli heat-shock factor, sigma32, revelas a multifaceted celular response toheat stress. Genes and Development, 20:1776-1789.
3. Cowing, D.H., Bardwell, J.C., Craig, E.A., Woolford, C., Hendrix, R.W. and Gross, C.A. (1985) Consensus sequence for Escherichia coli heat shock gene promoters. Proc. Natl. Acad. Sci USA, 82: 2679-2683.