Coding

Part:BBa_K762002:Design

Designed by: Menuka Samaranayake   Group: iGEM12_Wisconsin-Madison   (2012-09-24)

TCC Positive Control


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1361
    Illegal AgeI site found at 1473
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 685


Design Notes

The E0040 was amplified from the Bba_E0480 construct. This amplification took away the stop codon as well as the thymine base pair before the Spe1 site. This allows protein fusion between the GFP and TCC part. This fusion is needed for the TCC to function properly.

Source

2012 Distribution

References