Coding

Part:BBa_K759014:Design

Designed by: Kenta Mine   Group: iGEM12_HokkaidoU_Japan   (2012-09-25)


Codon optimized antigen43 (Ag43, agn43) for Escherichia coli K-12 strain


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 49
    Illegal AgeI site found at 259
    Illegal AgeI site found at 295
    Illegal AgeI site found at 493
    Illegal AgeI site found at 568
    Illegal AgeI site found at 607
    Illegal AgeI site found at 967
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This coding site contains BsrGI, NcoI and SacI restriction enzyme recognition sites. Because of difficulty of synthesizing whole sequence at a time, we planned to assemble this part by shortly synthesized parts with these enzymes.


Source

This coding site is referred to BBa_K346007 which was derived from E. coli K-12 strain.

References