Coding

Part:BBa_K759005

Designed by: Ami Kawahata   Group: iGEM12_HokkaidoU_Japan   (2012-09-19)

phaB (acetoacetyl-CoA reductase orgin from Ralstonia eutropha)

This gene codes the enzyme“acetoacetyl-CoA reductase” which catalyzes the reduction of acetoacetyl-CoA into (R)-3-hydroxybutyryl-CoA (3HB-CoA). It catalyzes the second step of P3(HB) producing pathway.
This gene was cloned from pGEM phbCAB which carries the synthetic operon genes (phaA,phaB,phaC) of Ralstonia eutropha.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 472
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 540


[edit]
Categories
//function/biosynthesis/plastic
Parameters
None