Regulatory

Part:BBa_K753000:Design

Designed by: Rachel Engeler   Group: iGEM12_Chalmers-Gothenburg   (2012-08-29)

Yeast FIG1 promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The length of the promoter was chosen to 450 bp as recommended by Kondo group of Kobe University of Japan.

Togawa S, Ishii J, Ishikura A, Tanaka T, Ogino C, Kondo A. Importance of asparagine residues at positions 13 and 26 on the amino-terminal domain of human somatostatin receptor subtype-5 in signalling. J Biochem. 2010 Jun;147(6):867-73


Source

The promoter has been amplified directly from genomic yeast DNA. Genomic upstream sequence was taken from Saccharomyces Genome Database: http://www.yeastgenome.org/cgi-bin/getSeq?map=amap&seq=S000000244&flankl=450&flankr=&rev=

References