Signalling

Part:BBa_K750115:Design

Designed by: Sifan Wang,Yizhen Yan,Ruosang Qiu,Youbin Mo   Group: iGEM12_XMU-China   (2012-09-22)

pBADcIT-PtetcIT-PcIGLT: arabinose or atc->cI protein->reduction of gfp(lva)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
    Illegal NheI site found at 1083
    Illegal NheI site found at 1106
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3686


Design Notes

This part has a right length but it can`t work.


Source

We built this part by using the biobricks from the dna distribution plates 2012.

References

[1]Tamsir, A., J.J. Tabor, and C.A. Voigt, Robust multicellular computing using genetically encoded NOR gates and chemical 'wires'. Nature, 2011. 469(7329): p. 212-5