Signalling

Part:BBa_K750105:Design

Designed by: Sifan Wang,Yizhen Yan,Jianzhao Chi,Qingshu Wu   Group: iGEM12_XMU-China   (2012-09-21)

pBADcIT-0.6:cI protein expression system activated by arabinose(RBS strength:0.6)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is designed to be a NOT gate for our poject.

Source

We built this part by using the biobricks from the dna distribution plates 2012.

References

[1]Tamsir, A., J.J. Tabor, and C.A. Voigt, Robust multicellular computing using genetically encoded NOR gates and chemical 'wires'. Nature, 2011. 469(7329): p. 212-5