Signalling

Part:BBa_K750001:Design

Designed by: Sifan Wang,Ruosang Qiu,Xinyi Yao,Qingshu Wu   Group: iGEM12_XMU-China   (2012-09-18)

LuxI expression device activated by arabinose(Regulated by RBS of 1.0 strength)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 793
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

By changing the RBS of LuxI producer, we can make AHL-LuxR spend different time to arrive the activation threshold of promoter LuxPR, and finally achieve our time delay goal.

Source

We built this part by biobricks from the DNA distribution kit plates 2012

References

[1]W.Claiborne Fuqua, S.C.W., E. Peter Greenberg, Quorum Sensing in Bacteria: the LuxR-LuxI Family of Cell Density-Responsive Transcriptional Regulatorst. JOURNAL OF BACTERIOLOGY, 1994. 176(2): p. 269-275.

[2]You, L., et al., Programmed population control by cell-cell communication and regulated killing. Nature, 2004. 428(6985): p. 868-71.

[3]Alon, U., Network motifs: theory and experimental approaches. Nat Rev Genet, 2007. 8(6): p. 450-61.

[4]Mangan, S., et al., The incoherent feed-forward loop accelerates the response-time of the gal system of Escherichia coli. J Mol Biol, 2006. 356(5): p. 1073-81.

[5]Camas, F.M., J. Blazquez, and J.F. Poyatos, Autogenous and nonautogenous control of response in a genetic network. Proc Natl Acad Sci U S A, 2006. 103(34): p. 12718-23.

[6]http://2011.igem.org/Team:XMU-China