Reporter

Part:BBa_K749025

Designed by: Takuya Akutsu   Group: iGEM12_TMU-Tokyo   (2012-09-26)

formaldehyde visualize and dehydrogenate device


Biosafety_Level1.png Safety.png


Formaldehyde monitoring and dehydrogenate device.
The upstream is sensor for formaldehyde and the downstream consecutively produces PFDH, formaldehyde dehydrogenase from Pseudomonas putida.
BBa_K749000, by itself, enables to observe the degree of formaldehyde decomposition with the naked eye.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 2231
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1239
    Illegal NheI site found at 1262
    Illegal PstI site found at 2231
    Illegal NotI site found at 1586
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 123
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 2231
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 2231
    Illegal NgoMIV site found at 1327
    Illegal NgoMIV site found at 1651
    Illegal NgoMIV site found at 1876
    Illegal NgoMIV site found at 2173
    Illegal AgeI site found at 1504
    Illegal AgeI site found at 1690
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1416
    Illegal BsaI.rc site found at 1011


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Categories
Parameters
None