Generator
Part:BBa_K749015:Experience
Designed by: Takuya Akutsu Group: iGEM12_TMU-Tokyo (2012-09-26)
E. coli transformated BBa_K749021 were cultured in LB medium overnight, disrupted by sonicator, and then extracted as the crude enzyme solution.
We mixed the emzyme solution 0.05 mL, 20 mM sodium formate 0.1 mL (2 mM final concentration), 20 mM NAD + 0.1 mL with 50 mM Tris-HCl pH 7.2 0.8 mL.
The mixture was incubated up to 30 mitutes and formate was quantified by liquid chromatography.
The details and result are described in our wiki.
only formate 2mM
Peak = 8.81(min)
BBa_K749015
| Reaction time | volume | Peak area | Peak area(%) |
| 30min | 50μl | 229371.2 | 80.88 |
| 10min | 20μl | 248294.2 | 83.9823 |
WT
| Reaction time | volume | Peak area | Peak area(%) |
| 10min | 20μl | 232791.6 | 83.691 |
No enzym
| Reaction time | volume | Peak area | Peak area(%) |
| 10min | 20μl | 243418.6 | 84.37 |
→http://2012.igem.org/Team:TMU-Tokyo/Notebook/Assay_3_Protocol_and_Result
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