Coding

Part:BBa_K749006:Design

Designed by: Takuya Akutsu   Group: iGEM12_TMU-Tokyo   (2012-09-26)


formate dehydrogenase 4 α-subunit from Methylobacterium extorquens


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2236
    Illegal PstI site found at 1948
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2236
    Illegal PstI site found at 1948
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2236
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2236
    Illegal PstI site found at 1948
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2236
    Illegal PstI site found at 1948
    Illegal NgoMIV site found at 1184
    Illegal NgoMIV site found at 1447
    Illegal NgoMIV site found at 1635
    Illegal NgoMIV site found at 1675
    Illegal NgoMIV site found at 1805
    Illegal NgoMIV site found at 2185
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 222
    Illegal BsaI site found at 1512
    Illegal BsaI.rc site found at 358
    Illegal BsaI.rc site found at 1051
    Illegal BsaI.rc site found at 1855
    Illegal SapI site found at 498
    Illegal SapI.rc site found at 60


Design Notes

None


Source

Methylobacterium extorquens

References