Reporter
sfGFP.degr

Part:BBa_K743018

Designed by:   Group: iGEM12_UC_Chile   (2012-09-23)

sfGFP.degradation.tag under Pta promoter in psb1C3_IntKR plasmid

This is a circadian clock controlled reporter for Synechocystis PCC6803. Placed in the psb1C3_IntKR integrative plasmid it has sfGFP with an C-terminal fast degradation tag under the control of the Pta promoter which comes from a Synechocystis gene known to oscillate in a circadian manner.

The short live of this tagged sfGFP allows for the caracterization of the activity of any promoter, in this case, the hypothesized oscillation of the Pta promoter.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2699
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2699
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2699
    Illegal XhoI site found at 3196
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2699
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2699
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 511
    Illegal SapI.rc site found at 699


[edit]
Categories
Parameters
None