Reporter
psb1C3_int

Part:BBa_K743016:Design

Designed by: Juan Simón Álamos   Group: iGEM12_UC_Chile   (2012-09-22)

psb1C3_IntKR recombination plasmid with RFP under psbA2 promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2551
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2551
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2551
    Illegal XhoI site found at 3048
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2551
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2551
    Illegal AgeI site found at 1164
    Illegal AgeI site found at 1276
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 511


Design Notes

This composite part is not intended to be used for standard biobrick assembly because of a EcoR1 restriction site in psb1C3_intK plasmid. You should use gibson assembly.


Source

psbA2 was amplified from Synechocystis genomic DNA and its sequence was obtained from BBa_K390017. RFP is from BBa_E1010.

For more information about the plasmid backbone, check psb1C3_IntKR recombination plasmid page.

References