Reporter
psb1C3_int

Part:BBa_K743016

Designed by: Juan Simón Álamos   Group: iGEM12_UC_Chile   (2012-09-22)


psb1C3_IntKR recombination plasmid with RFP under psbA2 promoter

The psb1C3_IntKR recombination plasmid allows the neutral integration of any desired sequence into Synechocystis PCC6803 chromosome. Composite part BBa_K743016 is designed for the expression of RFP under the control of the Synechocystis psbA2 promoter.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2551
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2551
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2551
    Illegal XhoI site found at 3048
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2551
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2551
    Illegal AgeI site found at 1164
    Illegal AgeI site found at 1276
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 511


[edit]
Categories
Parameters
None