Deleted
Sample Discontinued
Experience: Works
Not Used
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Plasmid
psb1C3_int

Part:BBa_K743007

Designed by:   Group: iGEM12_UC_Chile   (2012-09-20)

psb1C3_IntKR recombination plasmid for Synechocystis PCC6803

This plasmid allows the integration of a double terminator followed by a reversed Kanamycin resistance casette into Synechocystis PCC6803 chromosome to enable selection. It has a Chloramphenicol resistance casette for selection in E.coli.

The use of BBa_K743000 and BBa_K743001 as recombination sites has no deleterious phenotypic effects on the cells.

The plasmid is intended to be used as a starting backbone for further addition of DNA parts by Gibson assembly. This part was used by [http://2012.igem.org/Team:UC_Chile UC_Chile 2012 team] to succesfully transform Synechocystis PCC6803.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 511


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