Composite

Part:BBa_K741021:Design

Designed by: Wuyang Chen   Group: iGEM12_USTC-China   (2012-09-16)

plac-RBS-cI-T-pRM-anticro-T


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1370
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Different amount of anticro can be produced after different amount of IPTG is added to induce the promoter plac. The anticro is the antisense RNA of cro, which can inhibit the expression of cro.

Source

BBa_K741001 BBa_K741016

References