Composite

Part:BBa_K737040:Design

Designed by: Wenjie Wu and Peiran Zhang   Group: iGEM12_OUC-China   (2012-09-19)

galK::GFP generator ligated to TetR generator and constitutive sRNA device


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 1078
    Illegal NheI site found at 1101
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1890


Design Notes

No


Source

No

References

[1]Johannes H. Urban and Jo¨ rg Vogel, Translational control and target recognition by Escherichia coli small RNAs in vivo, Nucleic Acids Research, 2007, Vol. 35, No. 3

[2] Boris Görke and Jörg Vogel, Noncoding RNA control of the making and breaking of sugars, GENES & DEVELOPMENT 22:2914–2925 ,2008