Part:BBa_K733005
ybdN+Bmp2
This is mature Bone Morphogenetic Protein 2 (BMP2) with a singal peptide YbdN. BMP2 is a ligand involved in BMP pathway which belongs to TGF-β superfamily. It is a growth factor, which acts as a ligand, for inducing proliferation and differentiation of some type of cells. However, some cells, like colon cancer cell which is the target of HKUST-2012 IGEM team, would entre programmed apoptosis and kill themselves if the BMP2 is present (Beck et al, 2006). YbdN is a signal peptide in Bacillus subtilis. It is a signal peptide that allows the bacteria to secrete proteins to the extracellular environment. This signal peptide has high relative secretion activity (Tjalsma et al, 2000), compare with the signal peptide YdjM in another construct.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Reference
Beck, S. E., Jung, B. H., Fiorino, A., Gomez, J., Del Rosario, E., Cabrera, B. L., Huang, S. C., Chow, J. Y. C., & Carethers J.M. (2006). Bone morphogenetic protein signaling and growth suppression in colon cancer. The American Journal of Physiology-Gastrointestinal and Liver Physiology, 291(1), G135-G145.
Tjalsma, H., Bolhuis, A., Jongbloed, J. D. H., Bron, S., & Dijl, J. M. V. (2000). Signal peptide-dependent protein transport in Bacillus subtilis: a genome-based survey of the secretome. Microbiology and Molecular Biology Reviews, 64(3), 515-547.
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