Measurement

Part:BBa_K731702

Designed by: Giacomo Giacomelli, Anna Depetris   Group: iGEM12_UNITN-Trento   (2012-08-24)

BBa_K731700 backbone with BBa_K731722 E. coli terminator

This is the BBa_K731700 platform with the BBa_K731722 E. coli terminator inserted in the BioBrick cloning region. This part was used for the characterization of both BBa_K731722 and BBa_K731700.

K731702image.jpg

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 6850
    Illegal suffix found in sequence at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 6850
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 6856
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 6850
    Illegal BglII site found at 6011
    Illegal BamHI site found at 6832
    Illegal XhoI site found at 753
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 6850
    Illegal suffix found in sequence at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 6850
    Illegal XbaI site found at 6865
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NgoMIV site found at 714
    Illegal NgoMIV site found at 1051
    Illegal NgoMIV site found at 4231
    Illegal NgoMIV site found at 4391
    Illegal NgoMIV site found at 5979
    Illegal AgeI site found at 6800
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2228
    Illegal SapI.rc site found at 3310


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Categories
Parameters
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