Composite

Part:BBa_K731255

Designed by: Daniele Rossetto   Group: iGEM12_UNITN-Trento   (2012-08-03)

Inducible araC-pBAD promoter with GFPmut3b, weak RBS

This part has been used for testing and characterization of araC-pBAD promoter (BBa_K731201). The part is composed of part BBa_K731201 and BBa_E0240.

This part was successfully operated and characterized both in pSB1C3 and pSB3C5. Please head over to BBa_K731201 page for the characterization.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1876
    Illegal SapI site found at 961


[edit]
Categories
Parameters
None