Composite

Part:BBa_K727777:Design

Designed by: İBRAHİM YASİR ORHAN   Group: iGEM12_AUC_Turkey   (2012-06-16)

FMO3 Generator-Complete system


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 64
    Illegal suffix found in sequence at 1680
    Illegal PstI site found at 1233
    Illegal PstI site found at 1596
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 64
    Illegal SpeI site found at 1681
    Illegal PstI site found at 1233
    Illegal PstI site found at 1596
    Illegal PstI site found at 1695
    Illegal NotI site found at 70
    Illegal NotI site found at 1688
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 64
    Illegal BglII site found at 3130
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 64
    Illegal suffix found in sequence at 1681
    Illegal PstI site found at 1233
    Illegal PstI site found at 1596
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 64
    Illegal XbaI site found at 79
    Illegal SpeI site found at 1681
    Illegal PstI site found at 1233
    Illegal PstI site found at 1596
    Illegal PstI site found at 1695
    Illegal NgoMIV site found at 947
    Illegal AgeI site found at 447
    Illegal AgeI site found at 926
    Illegal AgeI site found at 1068
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Optimized for E.coli


Source

Genom of Human

References