Composite

Part:BBa_K727502:Design

Designed by: İBRAHİM YASİR ORHAN   Group: iGEM12_AUC_Turkey   (2012-06-16)

DMT Coding Device


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1053
    Illegal suffix found in sequence at 2474
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1053
    Illegal SpeI site found at 2475
    Illegal PstI site found at 2489
    Illegal NotI site found at 1059
    Illegal NotI site found at 2482
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1053
    Illegal BglII site found at 1177
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1053
    Illegal suffix found in sequence at 2475
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1053
    Illegal XbaI site found at 1068
    Illegal SpeI site found at 2475
    Illegal PstI site found at 2489
    Illegal AgeI site found at 1438
    Illegal AgeI site found at 1447
    Illegal AgeI site found at 2198
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 987


Design Notes

Optimized for E.coli


Source

Unknown

References