Composite

Part:BBa_K727500:Design

Designed by: İBRAHİM YASİR ORHAN   Group: iGEM12_AUC_Turkey   (2012-06-16)

TMT Coding Device


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1053
    Illegal suffix found in sequence at 2555
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1053
    Illegal SpeI site found at 2556
    Illegal PstI site found at 2570
    Illegal NotI site found at 1059
    Illegal NotI site found at 2563
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1053
    Illegal BglII site found at 2422
    Illegal BamHI site found at 1186
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1053
    Illegal suffix found in sequence at 2556
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1053
    Illegal XbaI site found at 1068
    Illegal SpeI site found at 2556
    Illegal PstI site found at 2570
    Illegal NgoMIV site found at 2131
    Illegal AgeI site found at 1403
    Illegal AgeI site found at 1981
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 987


Design Notes

Optimized for E.coli


Source

Unknown

References