Composite

Part:BBa_K727107:Design

Designed by: Mustafa Elitok   Group: iGEM12_AUC_Turkey   (2012-06-25)

GPPS coding device with GFP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 955
    Illegal suffix found in sequence at 1875
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 955
    Illegal SpeI site found at 1876
    Illegal PstI site found at 1890
    Illegal NotI site found at 961
    Illegal NotI site found at 1883
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 955
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 955
    Illegal suffix found in sequence at 1876
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 955
    Illegal XbaI site found at 970
    Illegal SpeI site found at 1876
    Illegal PstI site found at 1890
    Illegal NgoMIV site found at 1232
    Illegal AgeI site found at 1137
    Illegal AgeI site found at 1518
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 870


Design Notes

Optimized for E.coli


Source

Geraniol Producing Plants(Geranium, pelargonium etc.)

References