Generator

Part:BBa_K727002:Design

Designed by: Mustafa Elitok   Group: iGEM12_AUC_Turkey   (2012-06-16)

Dimethylamine Methyltransferase (DMT)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 1422
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 1423
    Illegal PstI site found at 1437
    Illegal NotI site found at 7
    Illegal NotI site found at 1430
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
    Illegal BglII site found at 125
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 1423
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 1423
    Illegal PstI site found at 1437
    Illegal AgeI site found at 386
    Illegal AgeI site found at 395
    Illegal AgeI site found at 1146
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Optimized for E.coli


Source

Unknown

References