Plasmid
pJEJAM14

Part:BBa_K678062:Design

Designed by: DTU-Denmark-2   Group: iGEM11_DTU-Denmark-2   (2011-09-13)

pJEJAM14


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 3726
    Illegal XbaI site found at 5282
    Illegal XbaI site found at 8026
    Illegal PstI site found at 140
    Illegal PstI site found at 2657
    Illegal PstI site found at 4567
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 910
    Illegal PstI site found at 140
    Illegal PstI site found at 2657
    Illegal PstI site found at 4567
    Illegal NotI site found at 5239
    Illegal NotI site found at 8065
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 144
    Illegal BamHI site found at 3007
    Illegal BamHI site found at 4305
    Illegal XhoI site found at 700
    Illegal XhoI site found at 1082
    Illegal XhoI site found at 4165
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 3726
    Illegal XbaI site found at 5282
    Illegal XbaI site found at 8026
    Illegal PstI site found at 140
    Illegal PstI site found at 2657
    Illegal PstI site found at 4567
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 3726
    Illegal XbaI site found at 5282
    Illegal XbaI site found at 8026
    Illegal PstI site found at 140
    Illegal PstI site found at 2657
    Illegal PstI site found at 4567
    Illegal NgoMIV site found at 3643
    Illegal AgeI site found at 1289
    Illegal AgeI site found at 1437
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4358
    Illegal BsaI.rc site found at 1116
    Illegal BsaI.rc site found at 3357
    Illegal SapI site found at 322


Design Notes

This plasmid was assembled with the Plug 'n' Play with DNA assembly standard (RFC 80), therefore this plasmid contains linkers between the biological parts as shown on the figure below.


DTU-Denmark-2 2011



Source

See the individual biobricks

References