Plasmid
pJEJAM13

Part:BBa_K678061:Design

Designed by: DTU-Denmark-2   Group: iGEM11_DTU-Denmark-2   (2011-09-13)

pJEJAM13


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 3777
    Illegal XbaI site found at 5333
    Illegal XbaI site found at 8077
    Illegal PstI site found at 140
    Illegal PstI site found at 4618
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 910
    Illegal PstI site found at 140
    Illegal PstI site found at 4618
    Illegal NotI site found at 5290
    Illegal NotI site found at 8116
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 144
    Illegal BamHI site found at 3058
    Illegal BamHI site found at 4356
    Illegal XhoI site found at 700
    Illegal XhoI site found at 1082
    Illegal XhoI site found at 4216
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 3777
    Illegal XbaI site found at 5333
    Illegal XbaI site found at 8077
    Illegal PstI site found at 140
    Illegal PstI site found at 4618
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 3777
    Illegal XbaI site found at 5333
    Illegal XbaI site found at 8077
    Illegal PstI site found at 140
    Illegal PstI site found at 4618
    Illegal NgoMIV site found at 3694
    Illegal AgeI site found at 1289
    Illegal AgeI site found at 1437
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4409
    Illegal BsaI.rc site found at 1116
    Illegal BsaI.rc site found at 3408
    Illegal SapI site found at 322


Design Notes

This plasmid was assembled with the Plug 'n' Play with DNA assembly standard (RFC 80), therefore this plasmid contains linkers between the biological parts as shown on the figure below.


DTU-Denmark-2 2011



Source

See the individual biobricks

References