Plasmid

Part:BBa_K678051:Design

Designed by: DTU-Danmark-2   Group: iGEM11_DTU-Denmark-2   (2011-09-13)

pJEJAM3


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1416
    Illegal EcoRI site found at 2294
    Illegal XbaI site found at 1459
    Illegal PstI site found at 874
    Illegal PstI site found at 1421
    Illegal PstI site found at 2384
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1416
    Illegal EcoRI site found at 2294
    Illegal PstI site found at 874
    Illegal PstI site found at 1421
    Illegal PstI site found at 2384
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1416
    Illegal EcoRI site found at 2294
    Illegal BglII site found at 1396
    Illegal BamHI site found at 1447
    Illegal XhoI site found at 1400
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1416
    Illegal EcoRI site found at 2294
    Illegal XbaI site found at 1459
    Illegal PstI site found at 874
    Illegal PstI site found at 1421
    Illegal PstI site found at 2384
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1416
    Illegal EcoRI site found at 2294
    Illegal XbaI site found at 1459
    Illegal PstI site found at 874
    Illegal PstI site found at 1421
    Illegal PstI site found at 2384
    Illegal NgoMIV site found at 3149
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This plasmid was assembled with the Plug 'n' Play with DNA assembly standard (RFC 80), therefore this plasmid contains linkers between the biological parts as shown on the figure below.


DTU-Denmark-2 2011



Source

See the individual biobricks

References