Other

Part:BBa_K678022:Design

Designed by: DTU-Denmark-2   Group: iGEM11_DTU-Denmark-2   (2011-09-04)


Neomycin cassette


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 591
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 591
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 591
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 591
    Illegal NgoMIV site found at 1042
    Illegal NgoMIV site found at 1325
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 891
    Illegal SapI.rc site found at 1101


Design Notes

Compatible with the Plug'n'Play assembly standard


Source

From plasmid pcDNA3.1 from Invitrogen

References