Other
hygR

Part:BBa_K678021

Designed by: DTU-Denmark-2   Group: iGEM11_DTU-Denmark-2   (2011-09-04)


Hygromycin cassette

Hygromycin cassette for selection of mammalian cells. The selection cassette contains the SV40 promoter, the hygromycin resistance gene, and the SV40 poly A as terminator

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 594
    Illegal PstI site found at 684
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 594
    Illegal PstI site found at 684
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 594
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 594
    Illegal PstI site found at 684
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 594
    Illegal PstI site found at 684
    Illegal NgoMIV site found at 1449
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/selectionmarker/antibioticresistance
//chassis/eukaryote/human
//direction/forward
Parameters
None