Composite

Part:BBa_K676008:Design

Designed by: Kheng Tee Ng   Group: iGEM11_UCL_London   (2011-09-17)

GyrA cassette


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1819
    Illegal BglII site found at 2764
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 253
    Illegal AgeI site found at 929
    Illegal AgeI site found at 986
    Illegal AgeI site found at 1625
    Illegal AgeI site found at 2381
    Illegal AgeI site found at 2749
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 269
    Illegal SapI.rc site found at 277
    Illegal SapI.rc site found at 1736


Design Notes

The wild type Gyr A ORF has a Pst 1 restriction site at the 1212th bp position so site directed mutagenesis has been carried out to remove the restriction site.


Source

Gyr A gene is obtained from E.coli genome.

References