Coding

Part:BBa_K669000:Design

Designed by: Nathaly Montenegro   Group: iGEM11_Colombia   (2011-09-28)


chiS


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1835
    Illegal EcoRI site found at 2420
    Illegal EcoRI site found at 2924
    Illegal SpeI site found at 2203
    Illegal SpeI site found at 3006
    Illegal PstI site found at 2861
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1835
    Illegal EcoRI site found at 2420
    Illegal EcoRI site found at 2924
    Illegal SpeI site found at 2203
    Illegal SpeI site found at 3006
    Illegal PstI site found at 2861
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1835
    Illegal EcoRI site found at 2420
    Illegal EcoRI site found at 2924
    Illegal BglII site found at 117
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1835
    Illegal EcoRI site found at 2420
    Illegal EcoRI site found at 2924
    Illegal SpeI site found at 2203
    Illegal SpeI site found at 3006
    Illegal PstI site found at 2861
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1835
    Illegal EcoRI site found at 2420
    Illegal EcoRI site found at 2924
    Illegal SpeI site found at 2203
    Illegal SpeI site found at 3006
    Illegal PstI site found at 2861
    Illegal AgeI site found at 340
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1288


Design Notes

Due to te presence of several restriction sites in the coding region we recommend to use this brick as a single plasmid.


Source

http://www.ncbi.nlm.nih.gov/gene?term=3279287%5Buid%5D

References