Coding

Part:BBa_K660300:Design

Designed by: Glasgow Uni 2011   Group: iGEM11_Glasgow   (2011-09-16)


Phosphodiesterase (enzyme)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 140
    Illegal PstI site found at 316
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 140
    Illegal PstI site found at 316
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 140
    Illegal PstI site found at 316
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 140
    Illegal PstI site found at 316
    Illegal NgoMIV site found at 253
    Illegal NgoMIV site found at 257
    Illegal NgoMIV site found at 268
    Illegal NgoMIV site found at 672
    Illegal NgoMIV site found at 1162
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 220


Design Notes

illegal restriction sites


Source

Amplified from Pseudomonas aeruginosa PA01

References