Coding

Part:BBa_K660200:Design

Designed by: Glasgow Uni 2011   Group: iGEM11_Glasgow   (2011-09-16)

Latherin (Surfactant Protein)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 200
    Illegal PstI site found at 431
    Illegal PstI site found at 569
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 200
    Illegal PstI site found at 431
    Illegal PstI site found at 569
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 514
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 200
    Illegal PstI site found at 431
    Illegal PstI site found at 569
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 200
    Illegal PstI site found at 431
    Illegal PstI site found at 569
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

Latherin was originally isolated from horse sweat. It was expressed in E.coli by researchers at University of Glasgow. We received it from them in a plasmid. We then amplified it up by PCR to add biobrick prefix and suffix.

References