Device
IR-3-GFP
Part:BBa_K658017:Experience
Designed by: Xin Wu Group: iGEM11_XMU-China (2011-10-02)
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Applications of BBa_K658017
The lux pR strength testing device BBa_K658017 with other lux pR strength testing devices BBa_K658016 BBa_K658018 BBa_K658019 was first cloned into plasmid pSB1A2 respectively, followed by transformation into E.coli strain BL21. Fluorescence was measured when cell growth reached a steady state (around 20h). The results are shown in following figures:
Figure 4 Promoters strength relative to lux pR-3 (BBa_K658006).
Figure 5 Efficiency of promoter lux pR (BBa_R0062) and its three mutants.
Figure 6: Fluorescence of four IR-GFP devices at 20h. 5, 3, 3/5, N represent for IR-3-GFP (BBa_K658017), IR-5-GFP (BBa_K658018), IR-3/5-GFP (BBa_K658019) and IR-GFP (BBa_K658016) respectively.
User Reviews
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