Coding

Part:BBa_K654060

Designed by: Cody Tramp   Group: iGEM11_Utah_State   (2011-09-26)

Fatty Acyl-CoA Reductase with 8-His Tag

Maqu_2507 Fatty Acyl CoA Reductase from Marinobacter aquaeolei (EC 1.2.1.50). Contains a 8-Histidine tag immediately before the stop codon to allow gel identification and extraction. The Fatty Acyl CoA Reductase converts fatty acyl-CoA into Fatty Alcohols.


2011 Utah State FattyAcylCoAReductase.png


Fatty Acyl-CoA Reductase is used to produce Fatty Alcohols, a valuable bioproduct, and these alcohols are used to produce Wax Esters, another valuable bioproduct in the 2011 Utah_State iGEM project.


2011 Utah State Pathway 2.png


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1709
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 659
    Illegal NgoMIV site found at 802
    Illegal AgeI site found at 25
    Illegal AgeI site found at 1424
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None