Composite

Part:BBa_K649207:Design

Designed by: Tomoyuki Ohno   Group: iGEM11_Tokyo_Tech   (2011-10-03)

PlacIQ-lox2272-gfp


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 107
    Illegal BamHI site found at 13
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 790


Design Notes

BBa_K649207 is composed of lox2272(BBa_K649204) and PlacIQ-gfp(BBa_J54202). BBa_J54202 contains Bgl II and Mlu I restriction site on upstream of gfp. These restriction sites allows insertion of the lox2272,designed to be flanked by the sticky ends of BamH I and Mlu I. This lox2272 was inserted to the PlacIQ-gfp digested with Bgl II and Mlu I.

Source

References

Construction of a genetic AND gate under a new standard for assembly of genetic parts. Ayukawa et al. BMC Genomics. 2010.