Other

Part:BBa_K648108:Design

Designed by: Alex Bina   Group: iGEM11_Penn_State   (2011-10-23)


RecA without restriction sites or recombinant activity


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 127
    Illegal AgeI site found at 631
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Activate RecA activity from RecA1, remove restriction sites and recombinant activity.


Source

The original strain of this part, RecA1, comes from the genomic sequence of the DH10B strain of Escherichia coli

References