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Part:BBa_K648102:Design

Designed by: Elyse Merkel   Group: iGEM11_Penn_State   (2011-09-28)


RecA with mutation of Lys 286


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 127
    Illegal AgeI site found at 631
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

RecA in activated form with muation at Lys 286 A->G in attempt to allow RecA to detect DNA and still cleave the CI not but recombinant damaged DNA


Source

E Coli RecA1 DH10B

References