Measurement

Part:BBa_K648034

Designed by: Lauren Rossi, Jamie Coletta, Anisha Katyal, Kristen Salava   Group: iGEM11_Penn_State   (2011-09-27)

Or tester

This part consists of the operating region (Or) for the lambda phage, and also a ribosome binding sight, mcherry with an rfp and a stop codon.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


We used this part to test that the promoter on the right side of Or was working properly. The production of red fluorescence protein was measured using a TECAN machine, and compared to a normal strand of E. coli. The data collected while the samples were in exponential growth was used to determine a rate of expression of the rfp. Compared to the control, the rate of fluorescence was much higher in our test subject, about 4227 compared to 418 (fu/OD600nm). This suggests that the promoter on Or is functional. A graph of the results is shown below.

PSUOrTest.png

OrTest2.jpg

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Categories
Parameters
n/aOr tester